![]() Select experiment to have Classifier retrieve objects from your entire experiment select image to retrieve objects from a particular image (you will be asked to type its ID number). After you Train Classifier (section III.C.5, following), new options will appear relating to each classification bin.įrom which images? Two system-supplied default values in the right-hand menu are experiment and image. Which class of objects should be retrieved? At this stage, random will be the only option available in the left-hand menu. How many objects? Enter the number of distinct objects you want Classifier to fetch (default = 20) ![]() You can also view the scores with CPA’s visualization tool, Plate Viewer, by using Database > Write Temporary Table in Database and running the Plate Viewer (section IV). You can save Classifier’s scores for each image (or group) from the Table Viewer using File > Save data to CSV or Save per-image counts to CSV to create comma-separated value files. You can double-click the headers of rows to view the corresponding images and then drag and drop objects from the resulting image(s) into classification bins to improve the classifier. You can click on column headings to sort the data by that column, helping you identify images that are highly enriched in a given object class, or images that simply have a high count of those objects. Classifier will present the results in a Table Viewer (described in section IV). Select File > Save Training Set from the menu bar (or ctrl+S).Ĭlick Score All to have Classifier score your entire experiment (optionally with groups or filters). It is advisable to do so before proceeding to scoring your experiment since scoring may take a long time for large screens. It is important to save the training set for future refinement, to re-generate scores, and as a record of your experiment. Objects can be dragged and dropped into bins from the Image Viewer or Image Gallery for further training. You will be able to specify that Classifier only retrieves objects that it deems to be in a particular class or objects that are difficult to classify so that you can correct errors.Ĭlick Score Image to visualize object classifications in a particular image (you will be asked to enter an image ID number). Repeat steps 2–5 to fetch and sort more objects. Bin names can be changed by right clicking empty space in the relevant bin.Įnter the number of top features you want to see (or if FastGentleBoosting is the chosen classifier, the maximum number of rules you want Classifier to look for). Often, two bins are used: positive and negative. Manually sort the unclassified objects into classification bins, adding additional bins if needed. n distinct objects will appear in the unclassified bin. Groups will only be available if defined in your properties file (section II).Ĭlick Fetch. Specify whether Classifier should select these objects from the entire experiment, a single image or a group, and whether it should apply any filters. Launch Classifier and enter the number of objects you want Classifier to fetch. Group (if you have defined groups in your properties file see section II.G), and computing theĮnrichment/depletion of each class per image or per group. ThisĮntails classifying all objects, counting how many objects of each class are in each image or ![]() Once classification reaches a desirable accuracy, Classifier can “score” your experiment. ![]() Rounds of refinement are necessary to train Classifier to recognize the That Classifier scores as being in a particular class īy fetching the objects predicted by Classifier to belong to a certain class and correcting errors in these classifications, subsequent Classifiers rapidly improve. Once Classifier is trained, you canĬontinue training by fetching and sorting either more random objects or those objects Once each binĬontains several example objects, you can start training Classifier on the annotated set, i.e., asking the machine to learn how toĭifferentiate the classes. Them into classification bins (representing object classes), to form an annotated set. You first request ( Fetch) object tiles (cropped from their original images), then manually sort User-supervised machine learning methods to object measurements. Classifier allows you to train the computer to identify objects of interest by applying iterative, ![]()
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